Related Experiment Video
Updated: Apr 21, 2026

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
Published on: April 17, 2017
Deuterium MAS NMR studies of dynamics on multiple timescales: histidine and oxalic acid
Monique Chan-Huot1, Stephen Wimperis, Christel Gervais
1Laboratoire des BioMolécules, UMR 7203, UPMC/ENS/CNRS, 24, rue Lhomond, 75231 Paris Cedex 05 (France); Sorbonne Universités, UPMC Univ Paris 06, LBM, 4 place Jussieu, 75005, Paris (France); École Normale Supérieure-PSL Research University, Département de Chimie, 24, rue Lhomond, 75005 Paris (France); Institut Curie, 91405 Orsay (France); INSERM U759, 91405 Orsay (France).
Abstract:
Deuterium ((2) H) magic-angle spinning (MAS) nuclear magnetic resonance is applied to monitor the dynamics of the exchanging labile deuterons of polycrystalline L-histidine hydrochloride monohydrate-d7 and α-oxalic acid dihydrate-d6 . Direct experimental evidence of fast dynamics is obtained from T1Z and T1Q measurements. Further motional information is extracted from two-dimensional single-quantum (SQ) and double-quantum (DQ) MAS spectra. Differences between the SQ and DQ linewidths clearly indicate the presence of motions on intermediate timescales for the carboxylic moiety and the D2 O in α-oxalic acid dihydrate, and for the amine group and the D2 O in L-histidine hydrochloride monohydrate. Comparison of the relaxation rate constants of Zeeman and quadrupolar order with the relaxation rate constants of the DQ coherences suggests the co-existence of fast and slow motional processes.
More Related Videos
08:40Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions
Published on: June 23, 2022
08:48High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
Published on: April 28, 2022
Related Concept Videos
¹H NMR of Labile Protons: Deuterium (²H) Substitution
¹H NMR of Labile Protons: Temporal Resolution
The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the...
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
2D NMR: Overview of Heteronuclear Correlation Techniques