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Updated: Apr 21, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

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Published on: April 7, 2014

Coded aperture pair for quantitative phase imaging.

Jiamin Wu, Xing Lin, Yebin Liu

|November 1, 2014

Summary

This summary is machine-generated.

This study introduces a new quantitative phase-imaging method using coded apertures for accurate light field reconstruction. It outperforms traditional methods, especially under partial coherence, without needing axial scanning.

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Last Updated: Apr 21, 2026

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Phase Contrast and Differential Interference Contrast DIC Microscopy

Phase Contrast and Differential Interference Contrast DIC Microscopy

Published on: August 6, 2008

Area of Science:

Optics and Photonics
Computational Imaging

Background:

Quantitative phase imaging is crucial for label-free microscopy.
Conventional methods like Transport-of-Intensity Equation (TIE) can be sensitive to illumination conditions and require axial scanning.

Purpose of the Study:

To develop a novel quantitative phase-imaging approach that enhances accuracy and robustness.
To eliminate the need for z-axial scanning in phase recovery.

Main Methods:

Optically encoding light fields into a complementary image pair using a coded aperture.
Computational reconstruction of the phase information from the image pair.
Utilizing the axial intensity derivative estimated from the coded-aperture image pair.

Main Results:

The proposed method accurately estimates the axial intensity derivative without z-axial scanning.
Achieved higher accuracy and robustness compared to conventional TIE-based methods.
Demonstrated effective performance under partial coherence illumination.

Conclusions:

The novel coded-aperture approach offers a robust and accurate alternative for quantitative phase imaging.
This method simplifies the experimental setup by removing the need for axial scanning.