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Updated: Apr 21, 2026

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Summary

Infrared laser ablation sample transfer (IR-LAST) is a new mass spectrometry technique. It enables ambient sampling and material transfer for detailed analysis of various samples, including tissues.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Laser Ablation

Background:

  • Ambient sampling techniques are crucial for mass spectrometry.
  • Traditional methods can be limited in sample preparation and analysis flexibility.

Purpose of the Study:

  • To introduce and evaluate Infrared Laser Ablation Sample Transfer (IR-LAST) for mass spectrometry.
  • To demonstrate IR-LAST's utility in tissue imaging with MALDI mass spectrometry.

Main Methods:

  • Utilized a pulsed mid-IR laser to ablate solid samples or deposited materials.
  • Transferred ablated material from tissue sections to target slides using transmission-mode IR laser ablation.
  • Analyzed transferred material using MALDI imaging mass spectrometry.

Main Results:

  • Achieved spatial resolution of approximately 400 μm, limited by plume spread.
  • Demonstrated ambient sampling capabilities.
  • Enabled area-to-spot concentration of ablated material.
  • Facilitated multiple ablations and analyses from a single sample section.
  • Allowed direct deposition on matrix-free nanostructured targets.

Conclusions:

  • IR-LAST offers a novel approach for ambient sample transfer in mass spectrometry.
  • The technique enhances capabilities for MALDI imaging, particularly for tissue analysis.
  • IR-LAST provides flexibility in sample handling and analysis concentration.