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Related Experiment Video

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Polarization second harmonic generation microscopy provides quantitative enhanced molecular specificity for tissue

Rajesh Kumar1, Kirsten M Grønhaug2, Elisabeth I Romijn3

  • 1Department of Physics, Norwegian University of Science and Technology (NTNU), N-7491, Trondheim, Norway. rajesh.kumar@ntnu.no, 101rajesh@gmail.com.

Journal of Biophotonics
|November 4, 2014
PubMed
Summary

Polarization second harmonic generation (p-SHG) microscopy differentiates biomolecules by analyzing light response. This technique quantifies molecular structure, aiding in pathological tissue diagnosis.

Keywords:
biomedical optical diagnosiscollagen fiberhuman knee articular cartilagemultiphoton microscopynonlinear optical imagingosteoarthritispolarization second harmonic generation (p-SHG) microscopysusceptibility (χ) tensor imaging

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Area of Science:

  • Biophysics
  • Molecular Imaging
  • Biomaterials Science

Background:

  • Biomolecules like collagen and myosin generate second harmonic generation (SHG) signals.
  • Molecular structure influences SHG signal response based on excitation light polarization.
  • Standard SHG intensity imaging cannot differentiate molecule types.

Purpose of the Study:

  • Implement polarization second harmonic generation (p-SHG) on commercial multiphoton microscopes.
  • Overcome artifacts in susceptibility (χ) imaging for accurate analysis.
  • Establish reference values for second-order χ tensor element ratios in various tissues.

Main Methods:

  • Utilized polarization-dependent second harmonic generation (p-SHG) microscopy.
  • Applied p-SHG to diverse tissue types for quantitative analysis.
  • Developed methods to mitigate artifacts in susceptibility (χ) imaging.

Main Results:

  • p-SHG successfully differentiates biomolecule types based on unique polarization responses.
  • Quantified the ratio of second-order χ tensor elements in different tissues.
  • Demonstrated collagen fiber type identification in ovarian tissue using χ tensor element ratios.

Conclusions:

  • p-SHG microscopy offers molecular-level differentiation beyond standard SHG imaging.
  • The ratio of second-order χ tensor elements serves as a potential biomarker for structural alterations.
  • p-SHG holds promise for pathological tissue diagnosis and structural analysis.