Development of amperometric glucose biosensor based on Prussian Blue functionlized TiO2 nanotube arrays

Area of Science:

• Electrochemistry
• Materials Science
• Biotechnology

Background:

• Amperometric biosensors utilizing oxidase and peroxidase enzymes are crucial for various applications.
• Titanium dioxide nanotube arrays (TiNTs) offer a promising platform for biosensor development due to their high surface area and electrochemical properties.

Purpose of the Study:

• To develop a novel enzymatic biosensor for glucose detection.
• To immobilize Prussian Blue (PB) as an artificial peroxidase and glucose oxidase (GOx) onto TiNTs.

Main Methods:

• PB nanocrystals were deposited onto TiNT walls using photocatalysis.
• GOx/AuNPs nanobiocomposites were immobilized within the nanotubes via polymer electrodeposition.
• The performance of the fabricated biosensor was evaluated for glucose sensing.

Main Results:

• The biosensor demonstrated a fast response, wide linear range, and good stability for glucose detection.
• Achieved high sensitivity of 248 mA M⁻¹ cm⁻² and a low detection limit of 3.2 μM.
• The integration of enzymes with TiNTs proved effective for bioelectrochemical applications.

Conclusions:

• The developed TiNT-based biosensor offers a promising strategy for sensitive and stable glucose monitoring.
• This approach provides a versatile platform for immobilizing various enzymes for biosensor and biofuel cell applications.
• The study highlights the potential of combining nanomaterials with enzymes for advanced bioelectrochemical devices.