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Updated: Apr 21, 2026

Pre-clinical Evaluation of Tyrosine Kinase Inhibitors for Treatment of Acute Leukemia
Published on: September 18, 2013
N-(2-amino-5-chlorobenzoyl)benzamidoxime derivatives inhibit human leukemia cell growth
Yukitoshi Nagahara1, Katsuhiko Nagahara2
1Division of Life Science and Engineering, College of Science and Engineering, Tokyo Denki University, Hatoyama, Hiki-gun, Saitama, Japan yuki@mail.dendai.ac.jp.
Background/Aim:
Amidoxime derivatives have been previously reported to have potent anti-microbial and anti-tumor activity. Little is known about the tumor cell growth-inhibition mechanism of amidoximes, especially benzamidoxime derivatives. Herein we determined the effects of N-(2-amino-5-chlorobenzoyl)benzamidoxime analogs on mammalian cancer cells.
Materials And Methods:
We synthesized four chloride-substituted benzamidoxime analogs from the original benzamidoxime to investigate their anticancer cell activity using the Jurkat T-cell lymphoma cell line and the human leukemia cell line HL-60RG.
Results:
All amidoxime derivatives inhibited Jurkat and HL-60RG cell viability dose-dependently. Benzamidoximes tended to damage HL-60RG cells to a greater extent compared to Jurkat cells. Benzamidoximes with chloride substitutes caused a strong decrease in cell growth, and this cell growth attenuation was transient at 5 μM (below the half-maximal inhibitory concentration, IC50) but long-lasting at 10 μM (greater than the IC50).
Conclusion:
Benzamidoxime derivatives caused a transient cell-cycle delay at a low dose and cell death at a high dose.
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