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Updated: Apr 21, 2026

Imaging Local Ca2+ Signals in Cultured Mammalian Cells
Published on: March 3, 2015
Multi-channel imaging of cellular signaling: interplay of Ca2+ and conventional protein kinase C
Peter Lipp1, Xin Hui1, Gergor Reither2
1Institute for Molecular Cell Biology and Research Center for Molecular Imaging and Screening, School of Medicine, Saarland University, 66421 Homburg/Saar, Germany;
Abstract:
To investigate the coupling between the calcium (Ca(2+)) signal and the conventional protein kinase C alpha (PKCα) translocation, which are both transient processes, a sectioning imaging technique with sufficient scanning speed is required. Here, we describe how to use a Nipkow-disk-based confocal system with an image splitter for two cameras to acquire simultaneously both the Ca(2+) signal and images of PKCα fused to the fluorescent protein DsRed2 in HEK293 cells on ATP stimulation.

