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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
Published on: January 7, 2019
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Rapid screening of peptide probes through in situ single-bead sequencing microarray
Weizhi Wang1, Zewen Wei, Di Zhang
1CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China , Beijing 100190, China.
Analytical Chemistry
|November 6, 2014
Summary
Researchers developed a rapid microfluidic method to screen peptide libraries for cancer targeting. This approach identified novel peptide ligands with high affinity and specificity for aminopeptidase N (APN), a tumor marker, paving the way for new diagnostics and therapeutics.
Area of Science:
- Biomedical engineering
- Chemical biology
- Molecular imaging
Background:
- Peptide ligands are valuable for in vivo imaging and drug delivery.
- Screening large peptide libraries for high affinity and specificity is challenging.
Purpose of the Study:
- To develop a rapid method for screening combinatorial peptide libraries.
- To identify novel peptide ligands targeting the tumor marker aminopeptidase N (APN).
Main Methods:
- Utilized a continuous-flow microfluidic system for one-bead-one-compound (OBOC) screening.
- Screened a library of 2 × 10(5) peptide beads in 4 hours.
- Employed the Clustal algorithm to identify conserved peptide sequences.
Main Results:
- Discovered 140 peptide hits targeting APN.
- Identified a conserved Tyr-XX-Tyr motif.
- Developed YVEYHLC peptides with nanomolar affinity and high specificity for APN in vitro and in vivo.
Conclusions:
- The microfluidic method enables rapid screening of peptide libraries.
- Novel peptide ligands targeting APN were identified.
- This technology facilitates the discovery of peptide-based reagents for disease diagnostics and therapeutics.
