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The defect of SFRP2 modulates an influx of extracellular calcium in B lymphocytes
Yuichi Tokuda, Masami Tanaka, Tomohito Yagi
1Department of Genomic Medical Sciences, Kyoto Prefectural University of Medicine, 465 kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan. tashiro@koto.kpu-m.ac.jp.
Background:
In the Wnt pathway, the secreted frizzled-related protein 2 (SFRP2) is thought to act as one of the several competitive inhibitors of Wnt. However, the precise role of SFRP2 is still poorly understood especially in B lymphocytes. Here, we investigated the function of SFRP2, comparing the SFRP2 defective as well as normal B lymphocytes in mice.
Results:
We demonstrated that calcium influx from extracellular to intracellular space in splenic B cells was clearly affected by the defect of SFRP2. In addition, the phosphorylation of phospholipase Cγ2 was observed to be reduced in SFRP2 defective splenic B cells with B cell receptor stimulation.
Conclusions:
SFRP2 is suggested to modulate the influx from extracellular calcium in the B cell receptor signaling pathway.
Insights
Secreted frizzled-related protein 2 (SFRP2) impacts calcium influx in B lymphocytes. SFRP2 deficiency alters B cell receptor signaling, affecting calcium levels and phospholipase Cγ2 phosphorylation.
Area of Science:
- Immunology
- Molecular Biology
- Cell Signaling
Background:
- Secreted frizzled-related protein 2 (SFRP2) is a Wnt pathway inhibitor.
- Its specific role in B lymphocytes remains largely unknown.
- This study investigates SFRP2 function in mouse B cells.
Purpose of the Study:
- To elucidate the function of SFRP2 in B lymphocytes.
- To compare SFRP2-deficient and normal B cells.
Main Methods:
- Comparative analysis of SFRP2-defective and normal mouse splenic B cells.
- Assessment of calcium influx.
- Evaluation of phospholipase Cγ2 phosphorylation upon B cell receptor stimulation.
Main Results:
- SFRP2 deficiency significantly affects extracellular calcium influx into splenic B cells.
- Phosphorylation of phospholipase Cγ2 is reduced in SFRP2-defective B cells following B cell receptor stimulation.
Conclusions:
- SFRP2 plays a role in modulating extracellular calcium influx within the B cell receptor signaling pathway.
- SFRP2 influences key signaling events in B lymphocytes.
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