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Related Experiment Videos

Veratrine-induced decrease of (Na+ + K+)-adenosine triphosphatase activity in rat brain slices.

T Matsuda1, H Iwata

  • 1Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.

The Journal of Pharmacology and Experimental Therapeutics
|February 1, 1989
PubMed
Summary
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Veratrine significantly decreases (Na+ + K+)-adenosine triphosphatase (ATPase) activity in rat brain slices, suggesting involvement of sodium-calcium exchange and calcium dependency in this inhibition.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Cellular Physiology

Background:

  • The (Na+ + K+)-adenosine triphosphatase (ATPase) is crucial for maintaining cellular ion gradients.
  • Membrane excitability can influence the function of ion-transporting enzymes like (Na+ + K+)-ATPase.
  • Understanding these interactions is vital for comprehending neuronal function and dysfunction.

Purpose of the Study:

  • To investigate the impact of membrane excitability on (Na+ + K+)-ATPase activity in rat brain.
  • To elucidate the mechanisms underlying veratrine-induced inhibition of (Na+ + K+)-ATPase.
  • To explore the role of ion concentrations and specific agents in modulating this enzyme's activity.

Main Methods:

  • Incubation of rat brain cortical slices with veratrine.

Related Experiment Videos

  • Assay of (Na+ + K+)-ATPase activity and its partial reactions.
  • Investigation of enzyme sensitivity to ouabain.
  • Experiments using modified ionic media (Na+-free, Ca++-free, high Mg++) and specific inhibitors (tetrodotoxin, amiloride, W-7, A23187).

    • Main Results:

    • Veratrine significantly inhibited (Na+ + K+)-ATPase activity in cortical, hippocampal, and hypothalamic slices.
    • The inhibition was independent of ouabain sensitivity, suggesting equal impact on enzyme isoforms.
    • Veratrine's effects were blocked by Na+-free medium or tetrodotoxin, and were dependent on external Ca++.
    • Sodium-calcium exchange and calcium influx appeared involved in the inhibitory mechanism.

    Conclusions:

    • Membrane excitability, induced by veratrine, leads to significant inhibition of (Na+ + K+)-ATPase activity in rat brain.
    • The inhibition mechanism involves calcium-dependent processes and potentially sodium-calcium exchange.
    • These findings highlight the intricate relationship between neuronal activity and ion pump function.