Related Experiment Video
Updated: Apr 21, 2026
08:08
Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis
Published on: February 19, 2018
9.1K
Control systems and coordination protocols of the secretory pathway
Alberto Luini1, Gabriella Mavelli2, Juan Jung3
1Consiglio Nazionale delle Ricerche (CNR), Istituto di Biochimica delle Proteine (IBP) Via Pietro Castellino 111, 80131 Napoli Italy ; Telethon Institute of Genetics and Medicine (TIGEM) Via Pietro Castellino 111, 80131 Napoli Italy.
F1000Prime Reports
|November 7, 2014
Summary
Cellular control systems manage the secretory pathway and Golgi complex for optimal function. Researchers are uncovering these hidden regulatory mechanisms using targeted perturbations.
Area of Science:
- Cell Biology
- Molecular Biology
Background:
- The secretory pathway and Golgi complex require regulatory systems for homeostasis.
- Coordination with other cellular modules like energy metabolism is essential.
Purpose of the Study:
- To investigate the control systems and coordination protocols governing the secretory pathway.
- To understand how these systems maintain cellular function during perturbations.
Main Methods:
- Utilizing controlled, transport-specific perturbations.
- Analyzing the responses to detect underlying regulatory devices.
Main Results:
- Emerging insights into the molecular components and architecture of secretory pathway control systems.
- Identification of coordination protocols linking the secretory apparatus with other cellular functions.
Conclusions:
- Regulatory devices are crucial for secretory pathway function but often hidden at steady state.
- Perturbation-based studies are key to revealing these essential cellular coordination mechanisms.
Related Concept Videos
01:33Overview of Secretory Vesicles
11.6K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
11.6K
01:17Role of ER in the Secretory Pathway
8.0K
Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
8.0K
01:28Protein Translocation Machinery on the ER Membrane
7.5K
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
7.5K
00:59COP Coated Vesicles
18.8K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
18.8K
01:45ER Retrieval Pathway
5.1K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
5.1K
01:22Protein Transport to the Thylakoids
3.2K
Thylakoids are membrane-bound sac-like structures within the chloroplast that serve as sites for photosynthesis. Thylakoid lumen contains many electron transport proteins and is enclosed by a thylakoid membrane rich in the light-harvesting complex. Proteins targeted to the thylakoids are transported as precursors and are sorted by the general TOC/TIC import pathway. Once the precursor reaches the stroma, stromal processing peptidases remove their transit signal and expose thylakoid signal...
3.2K