Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.9K
3.9K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

10.2K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.2K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

14.1K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
14.1K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

12.3K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
12.3K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

7.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.9K
RNA-seq03:21

RNA-seq

12.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Benchmarking DNA barcode decoding strategies under high error rates.

BMC bioinformatics·2026
Same author

A living biobank of sarcoma patient-derived cell cultures reveals multi-omic and functional insights that capture disease heterogeneity.

Clinical and translational medicine·2026
Same author

A RiboCancer cell line panel reveals that CLL-associated Rps15 mutations translationally rewire transcription through codon-specific tRNA accommodation defects.

HemaSphere·2026
Same author

Plasma cell-free transcriptome profiling in blood plasma from chronic liver disease patients.

Scientific data·2026
Same author

Expanding the human proteome with microproteins and peptideins.

Nature·2026
Same author

PeakPrime: a peak-guided primer design pipeline for target enrichment in 3'-end RNA-seq.

Bioinformatics advances·2026

Related Experiment Video

Updated: Apr 21, 2026

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

1.3K

An update on LNCipedia: a database for annotated human lncRNA sequences.

Pieter-Jan Volders1, Kenneth Verheggen2, Gerben Menschaert3

  • 1Center for Medical Genetics, Ghent University, Ghent 9000, Belgium.

Nucleic Acids Research
|November 8, 2014
PubMed
Summary
This summary is machine-generated.

LNCipedia 3.0 is a significantly expanded database of human long non-coding RNA (lncRNA) transcripts. It offers improved protein-coding potential assessment and gene conservation tools for researchers studying the non-coding genome.

More Related Videos

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.6K
Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
07:24

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

2.7K

Related Experiment Videos

Last Updated: Apr 21, 2026

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

1.3K
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.6K
Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
07:24

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

2.7K

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • The human genome produces thousands of non-coding RNA transcripts, predominantly long non-coding RNAs (lncRNAs).
  • The rapid identification of novel lncRNA genes necessitates comprehensive and updated repositories for annotation and analysis.
  • LNCipedia was established as an online resource to consolidate lncRNA information.

Purpose of the Study:

  • To present LNCipedia 3.0, the latest iteration of the human lncRNA database.
  • To enhance the assessment of protein-coding potential for lncRNA transcripts.
  • To implement a tool for evaluating lncRNA gene conservation across species.

Main Methods:

  • Database expansion incorporating over 90,000 new lncRNA transcripts.
  • Large-scale reprocessing of public proteomics data to refine protein-coding potential analysis.
  • Development of a comparative genomics tool for human, mouse, and zebrafish lncRNA genes.

Main Results:

  • LNCipedia 3.0 represents a five-fold increase in size compared to previous versions.
  • A high-confidence set of lncRNA transcripts with minimal coding potential has been identified and is available for download.
  • The new version includes an implemented tool for assessing lncRNA gene conservation.

Conclusions:

  • LNCipedia 3.0 provides a substantially enriched resource for studying human lncRNAs.
  • The improved coding potential assessment and conservation tools enhance the utility of the database for genomic research.
  • The database facilitates streamlined efforts in lncRNA discovery and characterization.