Related Experiment Videos
Interactions of Pseudorabies virus with swine alveolar macrophages I: virus replication
G Iglesias1, C Pijoan, T Molitor
1Department of Large Animal Clinical Sciences, College of Veterinary Medicine University of Minnesota, St. Paul.
Abstract:
The replication of Pseudorabies virus (PRV) in cultured swine alveolar macrophages (AM) was studied using 6 different virus strains. AM were highly permissive to PRV infection, with progeny virus titres of 10(7) TCID50/ml from some strains. Virus progeny titres were higher in cultures infected with the field strains S-62 and 4892 than in cultures infected with the strains Bartha or PRV-C. Virus replication, viral DNA synthesis and the concomitant cell damage were dependent upon virus input m.o.i.s. and virus strain. Furthermore, cells from 7 day old pigs yielded higher virus progeny titres than cells of 6 week old pigs. The results from this study provide support to the premise that PRV infection may predispose anials to respiratory disease.
Insights
Pseudorabies virus (PRV) replicates efficiently in swine alveolar macrophages (AM), with higher titers from field strains. Replication depends on virus strain, input dose, and piglet age, suggesting PRV may worsen respiratory disease.
Area of Science:
- Veterinary Virology
- Swine Immunology
- Infectious Disease Research
Background:
- Pseudorabies virus (PRV) is a significant pathogen in swine.
- Alveolar macrophages (AM) are crucial in respiratory defense against viral infections.
- Understanding PRV replication in AM is vital for disease management.
Purpose of the Study:
- To investigate the replication efficiency of different PRV strains in swine AM.
- To determine factors influencing PRV replication, including virus strain, input dose, and host age.
- To assess the potential of PRV infection to predispose pigs to respiratory disease.
Main Methods:
- Primary swine AM cultures were infected with six different PRV strains.
- Virus progeny titers were quantified using TCID50 assays.
- Viral DNA synthesis and cell damage were monitored.
- AM from pigs of different ages (7 days vs. 6 weeks) were used.
Main Results:
- Swine AM were highly permissive to PRV infection, with some strains achieving titers of 10(7) TCID50/ml.
- Field strains (S-62, 4892) showed higher progeny titers than attenuated strains (Bartha, PRV-C).
- Replication, DNA synthesis, and cell damage were dependent on virus strain and input multiplicity of infection (m.o.i.).
- Younger pigs (7-day-old) yielded higher virus progeny titers compared to older pigs (6-week-old).
Conclusions:
- Swine AM support efficient PRV replication, varying by virus strain and host factors.
- PRV replication dynamics are influenced by virus strain, m.o.i., and host age.
- PRV infection in pigs may increase susceptibility to secondary respiratory infections.