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Using the ubiquitous pH meter combined with a loop mediated isothermal amplification method for facile and sensitive
Shunbi Xie1, Yali Yuan, Yue Song
1State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education (Southwest University), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. yuanruo@swu.edu.cn yqchai@swu.edu.cn.
Abstract:
Here we show an amplification-coupled detection method for directly measuring released hydrogen ions during the loop mediated isothermal amplification (LAMP) procedure by using a pH meter. The genomic DNA of Nosema bombycis (N. bombycis) was amplified and detected by employing this LAMP-pH meter platform for the first time.
