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Related Concept Videos

Proteomics01:33

Proteomics

10.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.3K

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Related Experiment Video

Updated: Apr 21, 2026

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

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Large-scale label-free phosphoproteomics: from technology to data interpretation.

Hongbo Guo1, Ruth Isserlin, Andrew Lugowski

  • 1Banting & Best Department of Medical Research, Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, ON M5S, Canada.

Bioanalysis
|November 12, 2014
PubMed
Summary

This review covers high-performance MS/MS technology for identifying and quantifying phosphorylation sites. It details the pipeline from sample prep to data analysis for studying phosphoproteins in health and disease.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Proteomics

Background:

  • Protein phosphorylation is crucial for intracellular signaling and biochemical pathway control.
  • High-performance MS/MS technology enables large-scale identification and quantification of phosphorylation sites.

Purpose of the Study:

  • To review the complete data generation pipeline for phosphoprotein profiling.
  • To highlight challenges and future directions in interpreting phosphorylation networks.

Main Methods:

  • Sample preparation techniques.
  • Liquid Chromatography-Mass Spectrometry (LC-MS) detection.
  • Data processing and analysis software tools.

Main Results:

  • Systematic comparative profiling of thousands of phosphoproteins is achievable in single experiments.
  • The pipeline facilitates large-scale phosphoproteomic studies.

Conclusions:

  • Advances in MS/MS technology are revolutionizing the study of phosphorylation.
  • Mechanistic interpretation and visualization of phosphorylation networks are key for understanding human health and disease.