Biological sample collection for clinical proteomics: existing SOPs.

Area of Science:

• Biochemistry
• Clinical Proteomics
• Biomarker Discovery

Background:

• Proteomic studies of clinical samples are vital for understanding health and disease.
• Variations in body fluid proteomes can indicate physiological or pathological conditions.
• Accurate sample collection and preparation are critical for reliable proteomic data.

Purpose of the Study:

• To present current protocols and guidelines for human body fluid collection and storage for proteomic analysis.
• To offer a reliable guide for sample collection for proteomics investigators.
• To highlight the importance of standardized methods in clinical proteomics.

Main Methods:

• Review and compilation of existing protocols for human body fluid collection and storage.
• Presentation of guidelines for a variety of body fluids including blood, urine, CSF, saliva, and others.
• Emphasis on protocols reported by multiple independent research groups.

Main Results:

• A comprehensive overview of established protocols for collecting and storing diverse human body fluids for proteomic analysis.
• Detailed guidelines for commonly used fluids like plasma, serum, urine, and cerebrospinal fluid (CSF).
• Protocols for less common fluids such as nasal secretions, saliva, tears, and breast milk are also included.

Conclusions:

• Standardized collection and storage protocols are fundamental for advancing clinical proteomics.
• The presented guidelines serve as a reliable starting point for researchers in the field.
• Further refinement of these protocols is anticipated with technological advancements in proteomics.