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Isolation and functional assessment of cutaneous stem cells
Yanne S Doucet1, David M Owens
1Department of Dermatology, College of Physicians and Surgeons, Columbia University, New York, NY, 10032, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 13, 2014
Summary
This study details methods for isolating epidermal stem cells and assessing their function. It also highlights the crucial role of the skin
Area of Science:
- Dermatology
- Stem Cell Biology
- Tissue Renewal
Background:
- The epidermis is a multi-lineage tissue renewed by regionalized stem/progenitor cells.
- Recent advances allow isolation and functional assessment of epidermal stem cells.
- Stem cell progeny, homeostasis, and wound healing roles are key research areas.
Purpose of the Study:
- To provide a methodology for identifying and isolating epidermal stem cells.
- To assess the functional role of epidermal stem cells within their niche.
- To explore the influence of the microenvironment on epidermal stem cell function.
Main Methods:
- Identification and isolation of epidermal stem cells using molecular markers.
- Functional assessment of stem cells in their native niche.
- Analysis of microenvironmental cues (fibroblasts, adipocytes, neurons) influencing stem cells.
Main Results:
- Methodology provided for stem cell isolation and functional assessment.
- Demonstration of regionalized stem cell populations in epidermal homeostasis.
- Evidence of microenvironmental factors significantly impacting stem cell behavior.
Conclusions:
- Understanding epidermal stem cell niches is crucial for skin biology.
- Microenvironmental cues are vital for skin morphogenesis and homeostasis.
- Improved in vitro models are needed to study skin microenvironment interactions.
