Direct injection method for HPLC/MS/MS analysis of acrylamide in aqueous solutions: application to adsorption
Ines Mnif1, Charlotte Hurel, Nicolas Marmier
1ECOMERS, University Nice Sophia Antipolis, Parc Valrose, 28 Avenue Valrose, 06108, Nice Cedex 2, France, inesmnif@gmail.com.
Environmental Science and Pollution Research International
|November 13, 2014
Summary
A new HPLC/MS/MS method accurately detects toxic acrylamide in water. Acrylamide showed very weak adsorption to common environmental materials like clays and sludge.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
Background:
- Polyacrylamides are widely used polymers.
- They are a significant source of environmental acrylamide release.
- Acrylamide is a highly toxic compound.
Purpose of the Study:
- To develop a simple, rapid, and sensitive analytical method for acrylamide.
- To analyze acrylamide in water and adsorption samples.
- To investigate acrylamide adsorption on environmental matrices.
Main Methods:
- High-Performance Liquid Chromatography coupled with tandem Mass Spectrometry (HPLC/MS/MS) with direct injection.
- Method validation and uncertainty estimation using AFNOR standards.
- Adsorption studies on kaolinite, illite, and sludge.
Main Results:
- The developed method achieved a limit of quantification (LOQ) of 1 μg/L for acrylamide.
- Accuracy was confirmed at various concentration levels.
- Acrylamide adsorption on clays and sludge was consistently below 10%.
Conclusions:
- The developed HPLC/MS/MS method is suitable for sensitive acrylamide analysis.
- Acrylamide exhibits very weak adsorption to kaolinite, illite, and sludge.
- This low affinity is attributed to acrylamide's hydrophilic nature, small size, and charge neutrality.
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