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Changes in nuclear inositol phospholipids induced in intact cells by insulin-like growth factor I
L Cocco1, A M Martelli, R S Gilmour
1Istituto di Morfologia Umana Normale, University of Chieti, Italy.
Biochemical and Biophysical Research Communications
|March 15, 1989
Abstract:
Swiss 3T3 cells were labelled for 36 hours with high levels of 3H-myo-inositol and the radioactivity in nuclear inositol phospholipids was measured. Treatment of cells for 2 minutes, but not for 4 hours, with mitogenic concentrations of insulin-like growth factor I and bombesin caused a slight decrease in PtdIns and more marked decreases in PtdInsP and PtdInsP2. These effects were not seen if isolated nuclei were incubated with IGF-I and bombesin. We interpret these results to mean that rapid mass changes occur in nuclear inositol phospholipids in the early stages of the mitotic response.