Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Interference01:23

RNA Interference

29.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
29.0K
RNA Interference01:23

RNA Interference

7.9K
7.9K
Ribosome Profiling02:24

Ribosome Profiling

4.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.4K
Experimental RNAi02:15

Experimental RNAi

8.4K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D printing in core facilities - Low pain, high gain.

Journal of microscopy·2026
Same author

Stress and conflict management in core facilities-News from an imaging survey.

Journal of microscopy·2026
Same author

Comparative analysis of antibody-mediated loss-of-function versus gene knock-out and knock-down.

SLAS discovery : advancing life sciences R & D·2025
Same author

High-throughput screening of E3 ubiquitin ligases identifies TRIM48 as a novel negative regulator of RIG-I signaling.

Cellular signalling·2025
Same author

Targeted volume correlative light and electron microscopy of an environmental marine microorganism.

Journal of cell science·2023
Same author

Machine learning on large scale perturbation screens for SARS-CoV-2 host factors identifies β-catenin/CBP inhibitor PRI-724 as a potent antiviral.

Frontiers in microbiology·2023
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Apr 21, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

12.3K

Fluorescence microscopy-based RNA interference screening.

Manuel Gunkel1, Nina Beil, Jürgen Beneke

  • 1BioQuant, ViroQuant-CellNetworks RNAi Screening Facility, Ruprecht-Karls-Universitat Heidelberg, Im Neuenheimer Feld 267, Heidelberg, 69120, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|November 14, 2014
PubMed
Summary
This summary is machine-generated.

This study details automated methods for preparing cell culture plates for RNAi interference (RNAi) screening. The protocol enables efficient gene knockdown analysis in mammalian cells using siRNA transfection and microscopy.

More Related Videos

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

12.3K
RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
09:19

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

8.9K

Related Experiment Videos

Last Updated: Apr 21, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

12.3K
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

12.3K
RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
09:19

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

8.9K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biotechnology

Background:

  • RNA interference (RNAi) is a powerful tool for studying gene function by enabling gene knockdown in mammalian cells.
  • High-throughput screening requires efficient and reproducible methods for cell manipulation and analysis.

Purpose of the Study:

  • To present a protocol for the automated preparation of multiwell plates and cell arrays for RNAi screening.
  • To enable reverse transfection of individual wells/spots with specific small interfering RNA (siRNA) for gene knockdown studies.
  • To compare different microscopy techniques and discuss information workflow for screening approaches.

Main Methods:

  • Automated preparation of multiwell plates and cell arrays.
  • Reverse transfection of mammalian cells with siRNA.
  • Comparison of various microscopy types for screening.
  • Analysis of information workflow for high-throughput experiments.

Main Results:

  • Demonstration of a protocol for "ready to transfect" plates and cell arrays.
  • Facilitation of systematic gene knockdown studies using siRNA.
  • Evaluation of microscopy performance for screening applications.

Conclusions:

  • The presented protocol streamlines the process of RNAi screening in mammalian cells.
  • Automated preparation and standardized transfection enhance reproducibility and efficiency.
  • Considerations for microscopy and data handling are crucial for successful screening.