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Updated: Apr 21, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Practical structured illumination microscopy.

E Hesper Rego¹, Lin Shao

¹Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA, 02115, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 14, 2014

Summary

This summary is machine-generated.

Structured illumination microscopy (SIM) enhances fluorescence microscopy resolution by doubling it in 3D using structured light. This review details SIM principles, implementations, and nonlinear SIM for potentially unlimited resolution.

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Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

Area of Science:

Microscopy
Biophysics
Optical Imaging

Background:

Wide-field fluorescence microscopy offers limited spatial resolution.
Enhancing resolution is crucial for detailed biological imaging.
Structured illumination microscopy (SIM) provides a solution.

Purpose of the Study:

To introduce the fundamental principles of SIM.
To describe various SIM implementations.
To discuss nonlinear SIM and high-resolution imaging techniques.

Main Methods:

Utilizing spatially structured illumination light.
Employing diffraction gratings for illumination patterning.
Implementing liquid crystal spatial light modulators (SLMs).
Exploring nonlinear optical phenomena for resolution enhancement.

Main Results:

Achieved doubling of spatial resolution in three dimensions compared to conventional microscopy.
Demonstrated practical implementations of SIM using different optical setups.
Introduced nonlinear SIM as a method for potentially unlimited resolution.

Conclusions:

SIM is an effective technique for improving fluorescence microscopy resolution.
Diverse implementations cater to various imaging needs.
Nonlinear SIM holds promise for future super-resolution imaging advancements.