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Forskolin infusion in vivo increases ouabain binding in brain
1Department of Psychiatry, University of Texas Medical School, Houston 77225.
Abstract:
In order to investigate the possible regulation of brain Na,K-ATPase by cyclic AMP, we measured Na,K-ATPase activity and ouabain binding in cerebral cortex after intraventricular infusion of forskolin for 7 days. There was a dose-related increase in high-affinity ouabain binding, with a 75% increase at 12.5 micrograms/h forskolin. The effect on total enzyme activity was smaller but enzyme activity with high affinity for ouabain was increased by 65%, suggesting a selective effect on enzyme with high affinity for ouabain. Forskolin appeared not to interact directly with Na,K-ATPase in vitro.
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