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Expression of nuclear factor erythroid 2 protein in malignant cutaneous tumors

Chang Yong Choi1, Jin Young Kim1, Seo Yeong Wee1

  • 1Department of Plastic and Reconstructive Surgery, Soonchunhyang Gumi Hospital, Soonchunhyang University College of Medicine, Gumi, Korea.

Archives of Plastic Surgery
|November 15, 2014
PubMed
Abstract

Insights

Nuclear factor E2-related factor (Nrf2) protects against oxidative damage. This study found decreased Nrf2 protein expression in malignant melanoma, squamous cell carcinoma, and basal cell carcinoma, suggesting a link to skin cancer development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Dermatology

Background:

  • Reactive oxygen species (ROS) cause cellular damage and alter signaling.
  • Nuclear factor E2-related factor (Nrf2) is a key regulator protecting cells from oxidative stress.
  • Nrf2 is upregulated in many cancers, but its role in skin cancers is under-investigated.

Purpose of the Study:

  • To investigate the expression of Nrf2 protein in various human skin cancers.
  • To compare Nrf2 levels in malignant melanoma, squamous cell carcinoma, and basal cell carcinoma with normal skin tissues.

Main Methods:

  • Western blotting and immunohistochemical staining were used to analyze Nrf2 protein expression.
  • The study included 24 specimens: 6 malignant melanomas (MM), 6 squamous cell carcinomas (SCC), 6 basal cell carcinomas (BCC), and 6 normal skin samples.
  • Expression of Kelch-like ECH-associated protein 1 (Keap1), a key Nrf2 regulator, was also assessed.

Main Results:

  • Nrf2 protein was confirmed and detected in normal skin tissues.
  • Nrf2 expression was significantly decreased in all examined skin cancer types (MM, SCC, BCC) compared to normal skin.
  • Keap1 showed no expression in either malignant skin tumors or normal skin tissues via western blot.

Conclusions:

  • The study demonstrates down-regulation of Nrf2 protein in human malignant skin tumors.
  • Decreased Nrf2 expression is suggested to be associated with the pathogenesis of skin cancers.
  • Further research into the role of Nrf2 in skin cancer is warranted.
Keywords:
NF-E2-related factor 2Reactive oxygen speciesSkin neoplasms

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