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Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
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ARFGAP1 is dynamically associated with lipid droplets in hepatocytes
Joan Gannon1, Julia Fernandez-Rodriguez2, Hussam Alamri1
1The Research Institute of the McGill University Health Centre, McGill, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada.
Plos One
|November 15, 2014
Summary
ARF GTPase Activating Protein 1 (ARFGAP1) associates with lipid droplets and the Golgi apparatus. Its levels influence lipid droplet formation in cells and human liver tissue.
Area of Science:
- Cell Biology
- Molecular Biology
- Lipid Metabolism
Background:
- ARF GTPase Activating Protein 1 (ARFGAP1) is known to regulate vesicle formation at the Golgi apparatus.
- The role of ARFGAP1 in lipid metabolism and lipid droplet dynamics is not well understood.
Purpose of the Study:
- To investigate the association of ARFGAP1 with lipid droplets.
- To determine the effect of ARFGAP1 on lipid droplet formation and dynamics.
Main Methods:
- Cell culture experiments with oleate stimulation and cyclic AMP treatment.
- Overexpression and knockdown of ARFGAP1.
- Immunofluorescence microscopy of cultured cells and human liver tissue.
Main Results:
- ARFGAP1 transiently associates with lipid droplets upon oleate addition.
- Cyclic AMP treatment causes ARFGAP1 to shift from lipid droplets to the Golgi.
- Altering ARFGAP1 levels affects lipid droplet formation.
- ARFGAP1 is found associated with lipid droplets in some human hepatocytes.
Conclusions:
- ARFGAP1 plays a role in the dynamic regulation of lipid droplets.
- ARFGAP1 links Golgi vesicle trafficking to lipid droplet metabolism.
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