Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyphosphate-degrading enzymes in Acinetobacter spp. and activated sludge.

J W van Groenestijn1, M M Bentvelsen, M H Deinema

  • 1Department of Microbiology, Wageningen Agricultural University, The Netherlands.

Applied and Environmental Microbiology
|January 1, 1989
PubMed
Summary

This study investigated polyphosphate-degrading enzymes in Acinetobacter and activated sludge. Findings show a correlation between enzyme activity, phosphorus accumulation, and biological phosphate removal in wastewater treatment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacterial adhesion: A physicochemical approach.

Microbial ecology·2013
Same author

Comparison of Reductive Dechlorination of Hexachloro-1,3-butadiene in Rhine Sediment and Model Systems with Hydroxocobalamin.

Environmental science & technology·2011
Same author

Performance and population analysis of a non-sterile trickle bed reactor inoculated with Caldicellulosiruptor saccharolyticus, a thermophilic hydrogen producer.

Biotechnology and bioengineering·2008
Same author

Mite growth on fungus under various environmental conditions and its potential application to biofilters.

Experimental & applied acarology·2003
Same author

Biofouling reduction in recirculating cooling systems through biofiltration of process water.

Water research·2003
Same author

Optimum operation conditions of nitrogen and phosphorus removal by a biofilm-activated-sludge system.

Journal of environmental sciences (China)·2003

Area of Science:

  • Microbiology
  • Environmental Science
  • Biochemistry

Background:

  • Polyphosphate accumulation is crucial for biological phosphorus removal in wastewater treatment.
  • Acinetobacter species are key players in these microbial communities.
  • Understanding the enzymes involved is vital for optimizing wastewater treatment processes.

Purpose of the Study:

  • To characterize polyphosphate-degrading enzymes in Acinetobacter species and activated sludge.
  • To investigate the relationship between enzyme activity, growth rates, and phosphorus accumulation.
  • To correlate enzyme activities with biological phosphate removal efficiency.

Main Methods:

  • Enzyme assays were performed on Acinetobacter strains and activated sludge samples.
  • Polyphosphate: AMP phosphotransferase and polyphosphatase activities were measured.

Related Experiment Videos

  • Kinetic parameters (Km, Vmax) and optimal conditions (pH, temperature) were determined.
  • Correlations between enzyme activities, growth rates, and phosphorus content were analyzed.

    • Main Results:

    • Polyphosphate: AMP phosphotransferase activity in Acinetobacter decreased with higher growth rates.
    • Optimal conditions for this enzyme were pH 8.5 and 40°C, stimulated by ammonium sulfate.
    • Polyphosphatase activity was detected and strongly stimulated by high salt concentrations (NH4Cl).
    • A positive correlation existed between polyphosphate:AMP phosphotransferase, adenylate kinase activity, and phosphorus accumulation in Acinetobacter.
    • Adenylate kinase activity in activated sludge correlated with biological phosphate removal capacity.

    Conclusions:

    • Enzyme activities involved in polyphosphate metabolism vary with growth conditions in Acinetobacter.
    • Adenylate kinase activity serves as a reliable indicator for biological phosphate removal in activated sludge.
    • These findings contribute to understanding microbial phosphorus cycling in wastewater treatment.