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Published on: July 6, 2016
Synthesis of a Cu²⁺-selective probe derived from rhodamine and its application in cell imaging
Chunwei Yu1, Yingying Wen2, Jun Zhang3
1Laboratory of Environmental Monitoring, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, China. yucw_1979@sina.com.
Abstract:
A new fluorescent probe P based on rhodamine for Cu2+ was synthesized and characterized. The new probe P showed high selectivity to Cu2+ over other tested metal ions. With optimal conditions, the proposed probe P worked in a wide linear range of 1.0 × 10(-6)-1.0 × 10(-5) M with a detection limit of 3.3 × 10(-7) M Cu2+ in ethanol-water solution (9:1, v:v, 20 mM HEPES, pH 7.0). Furthermore, it has been used for imaging of Cu2+ in living cells with satisfying results.

