Knockdown of PSF1 expression inhibits cell proliferation in lung cancer cells in vitro
Jingyao Zhang1, Qifei Wu, Zhe Wang
1Department of Hepatobiliary Surgery, the First Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi'an, China.
Abstract:
Partner of sld five 1 (PSF1) is a member of the heterotetrameric complex termed GINS. Previous studies have shown that PSF1 is unregulated in several cancer and associated with tumor malignant characters. However, the effects of PSF1 in lung cancer are still unclear. The goal of this study was to investigate the effects of PSF1 on the proliferation capacities of lung cancer. To start with, expression of PSF1 in 22 human lung cancer samples and adjacent non-tumor samples were detected by real-time RT-PCR and Western blotting. Our results showed that PSF1 was overexpressed in lung cancer samples compared to adjacent non-tumor samples. To achieve better insights of PSF1 functions in lung cancer cells, we used PSF1-specific small interfering RNA (siRNA) successfully inhibit the expression of PSF1 in messenger RNA (mRNA) and protein levels. In addition, we used lung cancer cell lines with different p53 gene background (p53 null and p53 wild-type). The results showed that knockdown of PSF1 inhibited cell proliferation and caused cell cycle arrest of lung cancer cells in a p53-independent manner. Our data indicated that PSF1 is functionally involved in lung cancer cell proliferation and is a potential target for lung cancer therapy.
Insights
Partner of sld five 1 (PSF1) is overexpressed in lung cancer and drives tumor cell proliferation. Inhibiting PSF1 suppressed lung cancer growth independently of the p53 gene, suggesting PSF1 as a potential therapeutic target.
Area of Science:
- Molecular Biology
- Oncology
Background:
- Partner of sld five 1 (PSF1) is part of the GINS complex and implicated in various cancers.
- PSF1's role in lung cancer progression remains largely uncharacterized.
Purpose of the Study:
- To investigate the functional role of PSF1 in lung cancer proliferation.
- To determine if PSF1 is a viable therapeutic target for lung cancer.
Main Methods:
- Real-time RT-PCR and Western blotting to assess PSF1 expression in lung cancer tissues.
- PSF1 knockdown using small interfering RNA (siRNA) in lung cancer cell lines.
- Analysis of cell proliferation and cell cycle progression in p53-null and p53 wild-type cells.
Main Results:
- PSF1 was significantly overexpressed in human lung cancer tissues compared to adjacent non-tumor tissues.
- PSF1 knockdown via siRNA effectively reduced both mRNA and protein levels of PSF1.
- Inhibition of PSF1 suppressed lung cancer cell proliferation and induced cell cycle arrest in a p53-independent manner.
Conclusions:
- PSF1 plays a crucial role in promoting lung cancer cell proliferation.
- PSF1 functions independently of the p53 gene status in lung cancer cells.
- PSF1 represents a promising therapeutic target for lung cancer treatment.
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