Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fabrication of PCL Blended Highly Aligned Nanofiber Yarn from Dual-Nozzle Electrospinning System and Evaluation of the Influence on Introducing Collagen and Tropoelastin.

ACS biomaterials science & engineering·2025
Same author

A Biologic and Physical Characterization of an Injectable Amniotic Membrane Designed for Treating Diabetic Foot Ulcers.

Bioengineering (Basel, Switzerland)·2024
Same author

Arsenic Impairs Wound Healing Processes in Dermal Fibroblasts and Mice.

International journal of molecular sciences·2024
Same author

The Role of the Extracellular Matrix (ECM) in Wound Healing: A Review.

Biomimetics (Basel, Switzerland)·2022
Same author

An acellular tissue engineered biomimetic wound healing device created using collagen and tropoelastin accelerates wound healing.

Journal of tissue viability·2022
Same author

Improved Wound Closure Rates and Mechanical Properties Resembling Native Skin in Murine Diabetic Wounds Treated with a Tropoelastin and Collagen Wound Healing Device.

Journal of diabetes and clinical research·2021
Same journal

Gradient-Based Experimental Design for Defect Detection in MoS2 Including Emission Potentials for Thermal Diffuse Scattering.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

An Automated Atom Probe Tomography Cluster Detection Approach Using Transfer Learning.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Correlative Light and Electron Microscopy Visualization of Helicobacter pylori in Human Saliva.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Integrating Morpho-Anatomy and Histochemistry to Characterize Native Brazilian Eugenia Species.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Polyalthia Longifolia Induced Apoptosis via miR-484 Downregulation: A Multimodal In Situ Microscopy, In Vitro, and In Vivo Investigation.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Rhythmic Pattern of the Ovarian Development in Posthatching Japanese Quail (Coturnix coturnix japonica): Histological, Ultrastructural, and Immunohistochemical Study.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
See all related articles

Related Experiment Video

Updated: Apr 20, 2026

Dissection, MicroCT Scanning and Morphometric Analyses of the Baculum
04:32

Dissection, MicroCT Scanning and Morphometric Analyses of the Baculum

Published on: March 19, 2017

8.0K

Validating whole slide digital morphometric analysis as a microscopy tool.

Robert B Diller1, Robert S Kellar1

  • 11Department of Biological Sciences,Northern Arizona University,617 S. Beaver St.,P.O. Box 5640,Flagstaff,AZ 86011-5640,USA.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|November 18, 2014
PubMed
Summary
This summary is machine-generated.

Digital morphometric analysis (DMA) using whole slide imaging (WSI) and Feature Analysis on Consecutive Tissue Sections (FACTS®) provides results comparable to manual counting methods in histological analysis. This digital approach offers reproducible, unbiased, and high-throughput evaluation of tissue morphology.

Keywords:
whole slide quantitative analysis

More Related Videos

Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ
08:44

Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ

Published on: June 5, 2018

74.1K
Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps
08:59

Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps

Published on: October 28, 2018

7.7K

Related Experiment Videos

Last Updated: Apr 20, 2026

Dissection, MicroCT Scanning and Morphometric Analyses of the Baculum
04:32

Dissection, MicroCT Scanning and Morphometric Analyses of the Baculum

Published on: March 19, 2017

8.0K
Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ
08:44

Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ

Published on: June 5, 2018

74.1K
Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps
08:59

Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps

Published on: October 28, 2018

7.7K

Area of Science:

  • Histopathology
  • Digital Pathology
  • Morphometry

Background:

  • Whole slide imaging (WSI) enables quantitative histological analysis.
  • Manual morphometry is time-consuming and prone to bias.
  • Digital morphometric analysis (DMA) offers potential for automated quantification.

Purpose of the Study:

  • To compare the efficacy of DMA with manual counting methods.
  • To validate the use of Feature Analysis on Consecutive Tissue Sections (FACTS®) with WSI for quantitative analysis.
  • To assess the reproducibility and throughput of DMA.

Main Methods:

  • Utilized preserved rodent heart samples (n=14).
  • Sections were stained with Masson's trichrome and reacted for CD-68.
  • Digital morphometric analysis (DMA) was performed using FACTS® on WSI data.
  • Results were compared to manual counting methods.

Main Results:

  • No statistically significant difference was found between DMA and manual counting methods (p=0.38).
  • DMA provided similar quantitative results to manual assessment.
  • The study demonstrated the feasibility of DMA for histological analysis.

Conclusions:

  • DMA is a viable and accurate alternative to manual morphometry in histology.
  • DMA enhances reproducibility, reduces investigator bias, and increases throughput.
  • WSI combined with FACTS® offers a powerful tool for digital histological analysis.