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Related Experiment Video

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Assembling Molecular Shuttles Powered by Reversibly Attached Kinesins
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Computational modeling of kinesin stepping.

Hamidreza Khataee1, Alan Wee-Chung Liew

  • 1School of Information and Communication Technology, Gold Coast Campus, Griffith University , Gold Coast, Queensland 4222, Australia.

Journal of Chemical Information and Modeling
|November 18, 2014
PubMed
Summary

Kinesin motor proteins use adenosine triphosphate (ATP) hydrolysis and synthesis to control forward and backward stepping. Computational models reveal how ATP concentration and load influence kinesin

Area of Science:

  • Biophysics
  • Molecular Motors
  • Cell Biology

Background:

  • Kinesin is a crucial motor protein transporting cellular cargo along microtubules.
  • It functions as an information processor, converting cellular signals into mechanical actions.
  • Understanding kinesin's stepping mechanism is vital for cell motility and intracellular transport.

Purpose of the Study:

  • To develop a computational model for kinesin's mechanochemical kinetics.
  • To analyze the factors influencing kinesin's forward and backward stepping behavior.
  • To investigate the role of adenosine triphosphate (ATP) and load in kinesin's motor function.

Main Methods:

  • A computational model simulating kinesin as a digital circuit based on an ATP-driven finite state machine.

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  • Kinetic analysis of kinesin stepping under varying ATP concentrations and loads.
  • Simulation of kinesin's forward and backward stepping dynamics.

    • Main Results:

    • Backward stepping is primarily driven by ATP hydrolysis, while ATP synthesis prolongs this phase.
    • Kinesin pausing is influenced by ATP availability and load, with implications for motor velocity.
    • The computational model accurately replicates kinesin's stepping behavior across diverse conditions.

    Conclusions:

    • Kinesin's stepping dynamics are intricately regulated by ATP hydrolysis, synthesis, concentration, and external load.
    • The proposed finite state machine model provides a robust framework for understanding kinesin's information processing capabilities.
    • This study enhances our comprehension of molecular motor function and its regulation within the cell.