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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Scalable multiplexing for parallel imaging with interleaved optical coherence tomography.

Hee Yoon Lee1, Tahereh Marvdashti1, Lian Duan1

  • 1E.L. Ginzton Laboratory and Department of Electrical Engineering Stanford University, Stanford, CA 94305, USA.

Biomedical Optics Express
|November 18, 2014
PubMed
Summary
This summary is machine-generated.

We developed a faster optical coherence tomography (OCT) system using a virtually-imaged phased array (VIPA) to image 16 points simultaneously. This interleaved OCT (iOCT) achieves high-speed 3D imaging of biological samples.

Keywords:
(110.4500) Optical coherence tomography(110.6880) Three-dimensional image acquisition(170.3880) Medical and biological imaging(250.7260) Vertical cavity surface emitting lasers

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Area of Science:

  • Biomedical Optics
  • Optical Engineering
  • Imaging Technology

Background:

  • Interleaved optical coherence tomography (iOCT) enhances imaging speed by acquiring data from multiple spatial locations concurrently.
  • Virtually-imaged phased arrays (VIPAs) offer a method for spectral encoding, enabling parallel data acquisition.
  • Optimizing VIPA design is crucial for balancing multiplexing, ranging depth, and sensitivity in iOCT systems.

Purpose of the Study:

  • To demonstrate highly parallel imaging using an in-house-fabricated, air-spaced VIPA with iOCT.
  • To investigate the impact of different VIPA designs on the multiplexing capabilities of iOCT.
  • To showcase the system's performance for high-speed 3D imaging of biological specimens.

Main Methods:

  • Fabrication and integration of an air-spaced VIPA for spectral encoding of multiple lateral points.
  • Implementation of iOCT by multiplexing 16 lateral points onto a single wavelength sweep.
  • Experimental validation of system parameters and performance using a 200-kHz light source.

Main Results:

  • Achieved an effective A-scan rate of 3.2-MHz by multiplexing 16 lateral points.
  • Demonstrated tunable imaging parameters including the number of multiplexed points, ranging depth, and sensitivity.
  • Successfully performed 3D imaging of biological samples, including a human finger and a fruit fly, with improved sensitivity.

Conclusions:

  • The developed air-spaced VIPA enables highly parallel imaging, significantly increasing the effective A-scan rate for iOCT.
  • The system's flexibility in parameter tuning and improved sensitivity are suitable for advanced 3D biological imaging.
  • This approach offers a promising pathway for faster and more sensitive OCT-based investigations.