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Experimental infection of rhesus monkeys with SIV isolated from African green monkeys
O Herchenröder1, C Stahl-Hennig, W Lüke
1Deutsches Primatenzentrum, Göttingen, FRG.
Abstract:
To establish an animal model for AIDS, 6 juvenile rhesus monkeys were infected intravenously with cell-free SIVagmTYO-1, 5, or 7. One animal was infected with SIV of known pathogenicity isolated from a sooty mangabey (SIVsmm). The 2 animals infected with TYO-1, 1 of 2 infected with TYO-7 and the 1 infected with SIVsmm seroconverted within 4-8 weeks after infection and infectious virus could be recovered 8-10 or 44 weeks after infection. Three of the 4 seroconverted monkeys developed a persistent lymphadenopathy 12 weeks after infection. Rhesus monkeys infected with SIVagm could serve as a model to study HIV infection and for vaccine trials.
Insights
Rhesus monkeys infected with simian immunodeficiency virus (SIV) developed key AIDS-like symptoms. These SIV-infected monkeys serve as a valuable model for studying human immunodeficiency virus (HIV) infection and testing vaccines.
Area of Science:
- Veterinary Medicine
- Immunology
- Virology
Background:
- Establishing reliable animal models is crucial for understanding complex diseases like AIDS.
- Simian immunodeficiency virus (SIV) shares genetic and pathogenic similarities with human immunodeficiency virus (HIV).
Purpose of the Study:
- To evaluate the potential of rhesus monkeys as an animal model for acquired immunodeficiency syndrome (AIDS).
- To assess the infectivity and pathogenicity of specific SIVagm strains and SIVsmm in rhesus macaques.
Main Methods:
- Intravenous inoculation of 6 juvenile rhesus monkeys with cell-free SIVagmTYO-1, -5, or -7.
- Infection of one rhesus monkey with a pathogenic SIV isolated from a sooty mangabey (SIVsmm).
- Monitoring for seroconversion, viral recovery, and clinical signs such as lymphadenopathy post-infection.
Main Results:
- Two of three animals infected with SIVagmTYO-1 or SIVagmTYO-7 seroconverted within 4-8 weeks.
- One of two animals infected with SIVagmTYO-7 and the animal infected with SIVsmm also seroconverted.
- Persistent lymphadenopathy was observed in three of the four seroconverted monkeys 12 weeks post-infection.
Conclusions:
- Rhesus monkeys infected with SIVagm demonstrate potential as a relevant model for AIDS research.
- This SIV-infected rhesus monkey model is suitable for preclinical studies of HIV infection and vaccine efficacy trials.