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Synthesis of 11C-labelled thymidine for tumor visualization using positron emission tomography
E Poupeye1, R E Counsell, A De Leenheer
1Laboratoria voor Medische Biochemie en voor Klinische Analyse, Rijksuniversiteit Gent, Belgium.
International Journal of Radiation Applications and Instrumentation. Part A, Applied Radiation and Isotopes
|January 1, 1989
Abstract:
A no-carrier added synthesis of [11C]thymidine from cyclotron produced [11C]methyl iodide is presented. The bis-silylated derivative of the lithium salt of 5-bromo-2'-deoxyuridine is treated with [11C]methyl iodide in THF at -50 degrees C giving 20-30 mCi [11C]thymidine in a 40-70% radiochemical yield. Purification is performed by reversed phase chromatography on a C18 column. The whole procedure takes 25 min and the specific activity is about 20-30 mCi/mumol at the end of the synthesis.