Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyethylene glycol-attached antioxidant enzymes decrease pulmonary oxygen toxicity in rats.

C W White1, J H Jackson, A Abuchowski

  • 1Department of Pediatrics, University of Colorado School of Medicine, Denver 80262.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|February 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adipocyte-specific Nrf2 deletion negates nitro-oleic acid benefits on glucose tolerance in diet-induced obesity.

Nitric oxide : biology and chemistry·2024
Same author

A FABP4-PPARγ signaling axis regulates human monocyte responses to electrophilic fatty acid nitroalkenes.

Redox biology·2020
Same author

Evaluation of the reliability of the Cutaneous Dermatomyositis Disease Area and Severity Index and the Cutaneous Assessment Tool-Binary Method in juvenile dermatomyositis among paediatric dermatologists, rheumatologists and neurologists.

The British journal of dermatology·2017
Same author

Electrophilic nitro-fatty acids suppress allergic contact dermatitis in mice.

Allergy·2016
Same author

PEGylated Bovine Carboxyhemoglobin (SANGUINATE™): Results of Clinical Safety Testing and Use in Patients.

Advances in experimental medicine and biology·2016
Same author

Avoidance of Profound Hypothermia During Initial Reperfusion Improves the Functional Recovery of Hearts Donated After Circulatory Death.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2016

Polyethylene glycol (PEG)-attached superoxide dismutase and catalase (PEG-SOD + PEG-CAT) significantly extended survival in rats exposed to hyperoxia. PEG-SOD + PEG-CAT also reduced hyperoxia-induced lung injury.

Area of Science:

  • Biochemistry
  • Toxicology
  • Physiology

Background:

  • Hyperoxia (100% O2) induces acute oxidative lung injury.
  • Antioxidant enzymes like superoxide dismutase (SOD) and catalase (CAT) combat oxidative stress.
  • Polyethylene glycol (PEG) conjugation can modify enzyme properties.

Purpose of the Study:

  • To evaluate the protective effects of PEG-conjugated SOD and CAT against hyperoxia-induced lung injury in rats.
  • To compare the efficacy of PEG-enzymes with other antioxidant strategies.

Main Methods:

  • Rats were pretreated with PEG-SOD + PEG-CAT, saline, or other control solutions before continuous exposure to hyperoxia.
  • Survival rates and indicators of lung injury (lung oxidized glutathione, pleural effusions, albumin concentration) were assessed.

Related Experiment Videos

Main Results:

  • PEG-SOD + PEG-CAT pretreatment significantly increased rat survival time compared to saline or inactivated PEG-enzyme controls.
  • PEG-SOD + PEG-CAT treatment reduced markers of hyperoxia-induced lung injury.
  • PEG itself showed some hydroxyl radical scavenging activity in vitro.
  • PEG-enzymes were less protective than endogenous or liposomal antioxidant strategies.

Conclusions:

  • PEG-conjugated SOD and CAT offer protection against hyperoxia-induced lung injury and increase survival.
  • While PEG-enzymes provide a benefit, other antioxidant delivery methods may be more effective.
  • PEG may contribute to protection through direct radical scavenging properties.