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07:54
Cholesterol Efflux Assay
Published on: March 6, 2012
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Increased large VLDL particles confer elevated cholesteryl ester transfer in diabetes.
Robin P F Dullaart1, Rindert de Vries, Arjan J Kwakernaak
1Department of Endocrinology, University of Groningen and University Medical Center Groningen, Groningen, the Netherlands.
European Journal of Clinical Investigation
|November 18, 2014
Summary
Plasma cholesteryl ester transfer (CET) is elevated in Type 2 diabetes mellitus (T2DM). Large very low-density lipoprotein (VLDL) particles interact with T2DM to increase CET, contributing to atherosclerosis risk.
Area of Science:
- Lipid metabolism and cardiovascular disease research.
- Endocrinology and diabetes mellitus studies.
- Atherosclerosis and lipoprotein research.
Background:
- Plasma cholesteryl ester transfer (CET) reflects lipid movement from HDL to apoB lipoproteins, potentially promoting atherosclerosis.
- Elevated CET is observed in Type 2 Diabetes Mellitus (T2DM).
- The influence of T2DM on CET's relationship with lipoprotein subfractions requires elucidation.
Purpose of the Study:
- To investigate how T2DM modifies the association between plasma CET and very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL) subfractions.
- To determine the role of T2DM in the relationship between CET and specific lipoprotein particle concentrations and compositions.
Main Methods:
- Measured plasma CET, cholesteryl ester transfer protein (CETP) mass, and VLDL/LDL subfractions using nuclear magnetic resonance spectroscopy.
- Analyzed data from 62 T2DM patients and 53 non-diabetic subjects.
- Employed multivariable linear regression to assess interactions between T2DM status, glycemic control, and lipid parameters on plasma CET.
Main Results:
- T2DM patients exhibited increased plasma CET and CETP mass, associated with higher triglycerides and large VLDL particles.
- Plasma CET positively correlated with VLDL and LDL particle concentrations, independent of age, sex, and diabetes status.
- T2DM status significantly and independently interacted with VLDL concentration to influence plasma CET.
- Elevated plasma glucose and HbA1c further modified the CET-VLDL relationship.
- Specifically, T2DM status interacted positively with large VLDL particles to increase plasma CET.
Conclusions:
- Abnormalities in the concentration and composition of large VLDL particles contribute to elevated plasma CET in T2DM.
- These findings highlight the role of VLDL dysregulation in T2DM-associated lipid transfer abnormalities and atherosclerosis risk.
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