Magnetic resonance characterization of tissue engineered cartilage via changes in relaxation times, diffusion...

The primary goal of this paper is to describe a combined MR relaxation (T(2) and T(1ρ)), diffusion (apparent diffusion coefficient [ADC]), and elastography (shear stiffness) method of fully characterizing the development of tissue-engineered cartilage in terms of the changes in its composition, structure, and mechanical properties during tissue growth. Then, we may better use MR-based methodologies to noninvasively monitor and optimize the cartilage tissue engineering process without sacrificing the constructs. This process begins by demonstrating the potential capability of T(2), T(1ρ), ADC, and shear stiffness in characterizing a scaffold-free engineered cartilage. The results show that, in addition to the conventional T(2) and ADC, T(1ρ) and MRE can be used as potential biomarkers to assess the specific changes in proteoglycan content and mechanical properties of engineered cartilage during culture. Moreover, to increase the efficiency of MR characterization, two new methodologies for simultaneous acquisition of diffusion and MRE (dMRE), and T(1ρ) and MRE (T(1ρ)-MRE) are introduced that allow the simultaneous characterization of both biochemical and mechanical properties of engineered cartilage tissue. The feasibilities of dMRE and T(1ρ)-MRE approaches are validated on tissue-mimicking phantoms. The results show good correspondence between simultaneous acquisitions and conventional separate acquisition methods.