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DNA methylation: hemodialysis versus hemodiafiltration
Adrian-Bogdan Ghigolea1, Raluca Argentina Moldovan, Mirela Gherman-Caprioara
1"Iuliu Haţieganu" University of Medicine and Pharmacy, Department of Nephrology, Alba-Iulia, Alba, Romania; Alba-Iulia Nefromed Dialysis Center, Alba-Iulia, Alba, Romania; Alba-Iulia Emergency County Hospital, Alba-Iulia, Alba, Romania.
Summary
Aberrant DNA methylation is linked to chronic kidney disease and inflammation. On-line hemodiafiltration may offer a better DNA methylation profile compared to hemodialysis in dialysis patients.
Area of Science:
- Epigenetics
- Nephrology
- Molecular Biology
Background:
- Aberrant DNA methylation is increasingly recognized in chronic kidney disease (CKD).
- DNA methylation patterns are associated with inflammation in CKD patients.
- Dialysis techniques may influence epigenetic modifications in patients with kidney failure.
Purpose of the Study:
- To compare global DNA methylation status in dialysis patients undergoing different dialysis techniques.
- To investigate the relationship between DNA methylation, dialysis modality, and inflammation.
- To identify predictors of global DNA methylation in patients on dialysis.
Main Methods:
- Global DNA methylation was measured using a 5-mc DNA enzyme-linked immunosorbent assay in whole blood.
- Study included 80 dialysis patients (40 on-line hemodiafiltration, 40 high-flux hemodialysis) and 10 controls.
- Patients were stratified based on median 5-methylcytosine (5-mC) levels and inflammation status.
Main Results:
- Global DNA methylation was significantly higher in hemodialysis (HD) patients compared to on-line hemodiafiltration (HDF) patients and controls.
- Inflamed patients undergoing hemodialysis exhibited the highest levels of DNA methylation.
- Dialysis technique emerged as the sole independent predictor of global DNA methylation.
Conclusions:
- Global DNA methylation is elevated in dialysis patients, particularly those on hemodialysis.
- On-line hemodiafiltration may be associated with a more favorable DNA methylation profile.
- Dialysis modality is a key determinant of DNA methylation status in CKD patients.
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