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Intramembranous ossification is one of the two processes involved in the development of bones within an embryo. The flat bones of the face, most of the cranial bones, and the clavicles are formed via this process. During intramembranous ossification, the bones develop directly from sheets of undifferentiated mesenchymal connective tissue.
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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Osteoclast Derivation from Mouse Bone Marrow
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Osteoclast derivation from mouse bone marrow.

Ruth Tevlin1, Adrian McArdle2, Charles K F Chan3

  • 1Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine.

Journal of Visualized Experiments : Jove
|November 20, 2014
PubMed
Summary
This summary is machine-generated.

This study details a method for isolating and cultivating osteoclasts from mouse bone marrow. This protocol yields large numbers of osteoclasts, aiding research into bone diseases like osteoporosis.

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Area of Science:

  • Cell Biology
  • Skeletal Biology
  • Immunology

Background:

  • Osteoclasts are specialized bone-resorbing cells crucial for skeletal remodeling.
  • Dysfunctional osteoclasts contribute to bone diseases, including osteoporosis and neonatal lethal diseases.
  • Understanding osteoclast biology is key to developing treatments for skeletal pathologies.

Purpose of the Study:

  • To present a protocol for isolating and cultivating osteoclasts from mouse bone marrow.
  • To enable the generation of large quantities of osteoclasts for research purposes.
  • To facilitate further investigation into osteoclast function and related diseases.

Main Methods:

  • Isolation of bone marrow cells from mice.
  • Cultivation of monocyte/macrophage precursors.
  • Induction of differentiation into mature osteoclasts.

Main Results:

  • Successful isolation of osteoclasts from mouse bone marrow.
  • Generation of a high yield of functional osteoclasts in vitro.
  • The protocol provides a reliable method for obtaining osteoclasts for experimental use.

Conclusions:

  • The described protocol effectively isolates and cultivates osteoclasts.
  • This method supports advancements in understanding bone remodeling and disease.
  • It provides a valuable tool for discovering new therapeutic strategies for bone disorders.