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miR-103/107 modulates multidrug resistance in human gastric carcinoma by downregulating Cav-1

Ye Zhang1, Xiujuan Qu, Ce Li

  • 1Department of Medical Oncology, The First Hospital of China Medical University, NO. 155, North Nanjing Street, Heping District, Shenyang, 110001, China.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
|November 20, 2014
PubMed

Insights

MicroRNAs (miRNAs) like miR-103/107 can overcome multidrug resistance in gastric cancer. Upregulating miR-103/107 resensitizes cancer cells to doxorubicin by targeting caveolin-1.

Area of Science:

  • Molecular Biology
  • Oncology

Background:

  • MicroRNAs (miRNAs) are crucial regulators of biological processes, including cancer development.
  • Multidrug resistance (MDR) is a significant challenge in gastric cancer treatment.

Purpose of the Study:

  • To investigate the role of miRNA expression in multidrug-resistant gastric cancer cells.
  • To determine if miR-103/107 can overcome doxorubicin resistance in gastric cancer.

Main Methods:

  • MiRNA microarray analysis was used to compare miRNA expression between SGC7901/ADR and SGC7901 cells.
  • In vitro and in vivo drug sensitivity assays were performed to assess the effect of miR-103/107 overexpression.
  • Western blotting or similar techniques were used to confirm target interaction.

Main Results:

  • MiR-103/107 expression was found to be downregulated in multidrug-resistant SGC7901/ADR cells compared to parental SGC7901 cells.
  • Overexpression of miR-103/107 sensitized SGC7901/ADR cells to doxorubicin (DOX).
  • miR-103/107 was confirmed to inhibit P-glycoprotein (P-gp) function and target caveolin-1 (Cav-1).

Conclusions:

  • miR-103/107 plays a role in overcoming doxorubicin resistance in gastric cancer.
  • Restoring miR-103/107 levels may represent a therapeutic strategy to enhance chemosensitivity in gastric cancer.
  • Caveolin-1 is identified as a direct or indirect target mediating the effects of miR-103/107 on drug resistance.

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