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Updated: Apr 20, 2026
Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells
Published on: May 13, 2022
Improved ROS measurement in root hair cells
Silvina Paola Denita Juárez1, Silvina Mangano, José M Estevez
1Laboratorio de Fisiología y Biología Molecular, IFIByNE (CONICET), FCEyN, Universidad de Buenos Aires, Pab.II, Ciudad, Universitaria, Intendente Güiraldes 2160, Buenos Aires, C1428EGA, Argentina.
Reactive oxygen species (ROS) are vital plant signaling molecules involved in growth and stress responses. This study presents a protocol for detecting ROS using H2DCFDA, a fluorescein derivative, ensuring controlled cellular signaling.
Area of Science:
- Plant Biology
- Cell Signaling
- Biochemistry
Background:
- Reactive oxygen species (ROS) are crucial signaling molecules in plants.
- ROS are generated by NADPH oxidase in various cellular compartments.
- They regulate diverse processes including growth, defense, stress responses, and abscisic acid signaling.
Purpose of the Study:
- To describe a protocol for detecting ROS in plant cells.
- To enable the study of ROS involvement in plant processes.
Main Methods:
- Utilized the oxidation of 2',7-dichloro fluorescein (H2DCFDA), a fluorescein derivative.
- Developed a protocol for ROS detection in plant systems.
Main Results:
- The protocol allows for the detection of ROS.
- H2DCFDA serves as an effective indicator for ROS presence.
Conclusions:
- A reliable method for ROS detection is established.
- This protocol facilitates further investigation into the roles of ROS in plant physiology and signaling pathways.
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