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Coupling of viral membrane proteins to phosphatidylinositide signalling system
1Institut für Virologie und Immunbiologie, Universität Würzburg, FRG.
Abstract:
C6 rat glioma cells persistently infected with subacute sclerosing panencephalitis virus (C6/SSPE) were treated with measles antiserum and purified anti-measles IgG. This stimulated phosphoinositide breakdown and an increase in inositol phosphates. In uninfected C6 cells, however, only fetal calf serum (FCS), but not measles antiserum could induce inositol polyphosphate production.
Insights
Measles antiserum and IgG triggered phosphoinositide breakdown in virus-infected glioma cells. Uninfected cells only responded to fetal calf serum, not measles antiserum, indicating specific viral interactions.
Area of Science:
- Neurovirology
- Cellular signaling
Background:
- Subacute sclerosing panencephalitis (SSPE) is a persistent viral infection of the central nervous system.
- Understanding cellular responses to viral infections is crucial for developing therapeutic strategies.
Purpose of the Study:
- To investigate the effect of measles antiserum and anti-measles IgG on phosphoinositide metabolism in C6 rat glioma cells persistently infected with SSPE virus.
- To compare the cellular response in infected versus uninfected C6 cells.
Main Methods:
- Treatment of C6/SSPE cells and uninfected C6 cells with measles antiserum and purified anti-measles IgG.
- Measurement of phosphoinositide breakdown and inositol phosphate production.
Main Results:
- Measles antiserum and anti-measles IgG stimulated phosphoinositide breakdown and increased inositol phosphates in C6/SSPE cells.
- Uninfected C6 cells showed inositol polyphosphate production only when treated with fetal calf serum (FCS), not measles antiserum.
Conclusions:
- Measles-specific antibodies can modulate intracellular signaling pathways in virus-infected cells.
- The cellular response to immune components differs between infected and uninfected cells, suggesting virus-specific mechanisms.