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Cynomolgus Monkey Induced Pluripotent Stem Cells Generated By Using Allogeneic Genes
1Tsukuba Primate Research Center (TPRC), National Institute of Biomedical Innovation (NIBIO), 1-1 Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan. shimo@nibio.go.jp.
Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2014
Summary
Researchers created induced pluripotent stem (iPS) cells from cynomolgus monkey somatic cells using four key genes. These monkey iPS cells are valuable for biomedical research, avoiding complications from foreign transgenes.
Area of Science:
- Stem cell biology
- Primate research
- Genetic engineering
Background:
- Induced pluripotent stem (iPS) cells offer a potential alternative to embryonic stem (ES) cells.
- Generating species-specific iPS cells is crucial for biomedical research to avoid xenogeneic complications.
Purpose of the Study:
- To establish induced pluripotent stem (iPS) cells from cynomolgus monkey (Macaca fascicularis) somatic cells.
- To create a valuable resource for primate-based biomedical research.
Main Methods:
- Somatic cells from fetal liver and newborn skin of cynomolgus monkeys were utilized.
- Cells were infected with retroviral vectors containing cynomolgus monkey POU5F1, SOX2, KLF4, and c-MYC genes.
Main Results:
- Induced pluripotent stem (iPS) cells were successfully generated from cynomolgus monkey somatic cells.
- The generated iPS cells are similar to those derived from mouse and human cells.
Conclusions:
- The successful generation of cynomolgus monkey iPS cells using allogeneic transgenes is demonstrated.
- These monkey iPS cells represent a significant advancement for biomedical research, particularly in non-human primate models.
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