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Updated: Apr 20, 2026
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CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations
Published on: August 4, 2016
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Expression of human skin-specific genes defined by transcriptomics and antibody-based profiling.
Per-Henrik D Edqvist1, Linn Fagerberg2, Björn M Hallström2
1Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden (PHDE, AD, KE, FP)
Summary
This study identifies key genes and proteins specific to normal human skin using transcriptomics and protein profiling. These findings enhance our understanding of skin biology and potential disease biomarkers.
Area of Science:
- Dermatology
- Genomics
- Proteomics
Background:
- Understanding normal human skin molecular composition is crucial.
- Previous studies lacked comprehensive gene and protein expression profiles for skin.
Purpose of the Study:
- To define the molecular constituents of normal human skin.
- To characterize gene and protein expression landscapes in skin.
- To identify skin-specific genes and proteins.
Main Methods:
- Genome-wide transcriptomics analysis of skin biopsies.
- Deep sequencing of messenger RNA (mRNA).
- Immunohistochemistry-based protein profiling.
- Comparison of skin data with 26 RNA and 44 protein tissue types.
Main Results:
- Identified 417 genes with elevated expression in skin.
- Discovered 106 genes expressed at least five-fold higher in skin compared to other tissues.
- Highlighted C1orf68 as a highly skin-enriched protein.
- Mapped precise localization of identified proteins within skin compartments.
Conclusions:
- Successfully identified the human skin-specific proteome.
- Facilitated further functional studies of skin molecular repertoire.
- Aimed to identify potential biomarkers for various skin diseases.
