Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intermetallic reaction and eutectic transitions tune the reactivity in core-shell Mg/Ni nanoparticles.

Physical chemistry chemical physics : PCCP·2026
Same author

Dual-functional tea polyphenol-serotonin nanoparticles for integrated inflammation suppression and mucosal repair in inflammatory bowel disease.

Biomaterials·2026
Same author

Histone Deacetylase Inhibitor Entinostat Exerts Anti-NSCLC Effects Through the EGFR Signaling Pathway and MDM2-p53 Axis.

Current pharmaceutical biotechnology·2026
Same author

Interface Reaction in Core-Shell Boron-FeF<sub>3</sub>: Direct Observation of Gas Release and Oxide Removal during Combustion.

ACS applied materials & interfaces·2026
Same author

A generative spike prediction model using behavioral reinforcement for re-establishing neural functional connectivity.

Nature computational science·2026
Same author

Microscopic Insight into Knudsen and Electromagnetic Effects on Thermal Conductivity of Closed Mesoporous Metal Gels.

Gels (Basel, Switzerland)·2025
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
Same journal

Tunable Charge Transfer in Europium Metal-Organic Frameworks for Ratiometric Sensing of a Sarin Simulant.

Analytical chemistry·2026
Same journal

A β-Cyclodextrin/Ag<sub>2</sub>O@MWCNT-Based Stochastic Platform for the Simultaneous Molecular Enantiorecognition and Enantioanalysis of Twelve Amino Acids in Biological Matrices.

Analytical chemistry·2026
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Apr 20, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.8K

Absolute quantification method for protein concentration.

Mingdong Li1, Jiaojie Tan, Michael J Tarlov

  • 1Department of Chemical and Biomolecular Engineering, University of Maryland , College Park, Maryland 20742, United States.

Analytical Chemistry
|November 21, 2014
PubMed
Summary
This summary is machine-generated.

This study introduces a novel assay for precise protein concentration measurement using electrospray differential mobility analysis (ES-DMA). The method quantifies protein oligomers without needing protein-specific calibration, offering a universal solution.

More Related Videos

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB
08:10

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB

Published on: August 21, 2018

11.7K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.9K

Related Experiment Videos

Last Updated: Apr 20, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.8K
High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB
08:10

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB

Published on: August 21, 2018

11.7K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.9K

Area of Science:

  • Analytical Chemistry
  • Biophysics
  • Biotechnology

Background:

  • Accurate protein quantification is crucial for biological research and drug development.
  • Existing methods often require protein-specific calibration, limiting their universal applicability.
  • Electrospray ionization is a common technique for analyzing biomolecules.

Purpose of the Study:

  • To develop a fast and accurate assay for determining the absolute concentration of proteins.
  • To demonstrate the utility of electrospray differential mobility analysis (ES-DMA) for protein quantification.
  • To eliminate the need for protein-specific calibration in concentration measurements.

Main Methods:

  • Direct measurement of droplet-entrapped oligomer formation in electrospray.
  • Utilizing electrospray differential mobility analysis (ES-DMA) to differentiate monomers, dimers, and higher-order oligomers.
  • Applying the method to various protein samples including NIST Standard Reference Material 927e, immunoglobulin G 1κ, and Rituximab.

Main Results:

  • The ES-DMA method successfully distinguished between different protein oligomeric states.
  • Absolute protein number concentrations were determined without protein-specific calibration.
  • The assay demonstrated accuracy and speed in quantifying diverse protein samples.

Conclusions:

  • The described assay provides a universal and calibration-free method for absolute protein concentration determination.
  • ES-DMA is a powerful tool for analyzing protein oligomerization and concentration.
  • This technique has broad applicability across various electrospray-based analytical platforms.