Related Experiment Videos
Antibodies specific to the retinoic acid human nuclear receptors alpha and beta
1Unité 184 de Biologie Moléculaire et de Génie Génétique de Institut National de la Santé et de la Recherche Médicale, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
Abstract:
Two cDNAs encoding two human receptors for retinoic acid (RA), RAR-alpha and RAR-beta, have been characterized recently. Synthetic peptides corresponding to the cDNA-deduced amino acid sequences unique to RAR-alpha and RAR-beta were used to generate anti-RAR-alpha antiserum (SP171) and anti-RAR-beta antisera (SP172 and SP248). The specificity of these antisera was confirmed both by immunocytochemical detection of these receptors in COS-1 cells transfected with RAR-alpha and RAR-beta expression vectors and by immunoblot analyses performed with whole extracts of these cells. We also demonstrate that these antisera recognize RAR-alpha and RAR-beta endogenously expressed in the RA-responsive human promyelocytic leukemia cell line HL-60.