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Updated: Apr 20, 2026
Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection
Published on: May 10, 2024
A novel acute necrotizing pancreatitis model induced by L-arginine in rats
Fei Guo1, Shutao Zheng, Xiaokang Gao
1From the *Department of Emergency Surgery, and †Clinical Medical Research Institute, State Key Lab Breeding Base of Xinjiang Major Diseases Research, the First Affiliated Hospital of Xinjiang Medical University; and ‡Department of Urology, and §Department of Hepatobiliary Surgery, Urumqi General Hospital of Lanzhou Military Region, Urumqi, Xinjiang, People's Republic of China.
Objective:
This study developed a novel protocol for creating an acute necrotizing pancreatitis model in rats using L-arginine.
Methods:
Anesthetic laparotomy was performed on the upper abdomen, and the pancreatic parenchyma of Sprague-Dawley rats was injected with 1 mL of sterilized L-arginine solution at 5 different locations in the experimental group. Specifically, 2 different injection points in the head and body of the pancreas were chosen randomly, and 1 injection point in the tail of the pancreas was chosen randomly. The parenchyma of the pancreas was injected with 200 μL of an L-arginine solution at each point. The optimal dose of L-arginine per rat was 0.4 g/kg.
Results:
Serum amylase activity increased significantly after targeted injection into the parenchyma of the pancreas. Pathological examination of the pancreas 24 hours after L-arginine injection revealed massive interstitial edema, apoptosis, and necrosis of acinar cells with an infiltration of neutrophils, granulocytes, and monocytes.
Conclusions:
The present study developed an appropriate, workable, and reproducible rat model of acute necrotizing pancreatitis with higher survivability and success rates compared with previously published methods.
