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Related Experiment Video

Updated: Dec 26, 2025

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RUNX3 Inhibits the Invasion and Metastasis of Human Colon Cancer HT-29 Cells by Upregulating MMP-2/9.

Jun Xue1, Xueliang Wu1, Ming Qu1

  • 1Department of General Surgery, First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China.

Evidence-Based Complementary and Alternative Medicine : Ecam
|March 19, 2020
PubMed
Summary

Runt-associated transcription factor 3 (RUNX3) inhibits colon cancer cell invasion and metastasis. This is linked to reduced expression of matrix metalloproteinases (MMP)-2 and MMP-9, suggesting a potential therapeutic target.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Colon cancer is a leading cause of cancer-related deaths worldwide.
  • Understanding the molecular mechanisms driving colon cancer invasion and metastasis is crucial for developing effective treatments.
  • Runt-associated transcription factor 3 (RUNX3) is a tumor suppressor gene implicated in various cancers.

Purpose of the Study:

  • To investigate the effect of RUNX3 on the invasion and metastasis of human colon cancer HT-29 cells.
  • To explore the preliminary mechanism of RUNX3's anticancer effect in colon cancer.

Main Methods:

  • Human colon cancer HT-29 cells were transfected with a RUNX3 plasmid vector.
  • Gene expression of RUNX3 and matrix metalloproteinases (MMP)-2/9 was analyzed using RT-PCR and western blot.
  • Cell proliferation, invasion, and migration were assessed using MTT assay, scratch injury assay, Transwell chamber, and Matrigel invasion models.

Main Results:

  • Stable RUNX3 expression was achieved in HT-29 cells.
  • RUNX3 overexpression significantly reduced the expression of MMP-2 and MMP-9 mRNA and proteins.
  • RUNX3 transfection led to decreased cell proliferation, invasion, and migration capabilities.

Conclusions:

  • RUNX3 effectively inhibits the invasion and metastasis of human colon cancer HT-29 cells.
  • The mechanism involves the downregulation of MMP-2 and MMP-9 expression.
  • RUNX3 represents a potential therapeutic target for colon cancer treatment.