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Related Concept Videos

iPS Cell Differentiation01:22

iPS Cell Differentiation

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The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
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Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
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Hepatic Differentiation from Human Ips Cells Using M15 Cells.

Kahoko Umeda1,2, Nobuaki Shiraki1, Shoen Kume3

  • 1Department of Stem Cell Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Honjo 2-2-1, Chuo-ku, 860-0811, Kumamoto, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|November 24, 2014
PubMed
Summary

This study demonstrates efficient differentiation of human induced pluripotent stem cells (hiPSCs) into hepatocytes using a mesonephros-derived cell line (M15). This method yields albumin-producing liver cells, valuable for drug testing and disease research.

Keywords:
Endoderm differentiationFeeder cellsHepatic differentiationM15 cells

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Area of Science:

  • Stem Cell Biology
  • Hepatology
  • Developmental Biology

Background:

  • Human induced pluripotent stem cells (hiPSCs) offer a promising source for regenerative medicine.
  • Efficient differentiation protocols are crucial for generating functional cell types for research and therapeutic applications.
  • Liver disease research requires reliable in vitro models for drug screening and mechanistic studies.

Purpose of the Study:

  • To establish a simple and efficient method for differentiating hiPSCs into functional hepatocytes.
  • To utilize a mesonephros-derived cell line (M15) as a feeder system for hepatic differentiation.
  • To assess the efficiency of generating albumin-expressing and albumin-secreting hepatocytes from hiPSCs.

Main Methods:

  • Human iPS cells were cultured and differentiated using the M15 cell line as a feeder layer.
  • Immunofluorescence staining was performed to identify SOX17-positive definitive endoderm and albumin-positive hepatocytes.
  • Albumin secretion was measured to confirm hepatocyte functionality.

Main Results:

  • Approximately 90% of hiPSCs successfully differentiated into SOX17-positive definitive endoderm.
  • Around 50% of the differentiated cells expressed albumin and secreted ALB protein, indicating functional hepatocytes.
  • The M15 feeder system proved to be a simple and effective method for hepatic differentiation.

Conclusions:

  • The M15 feeder system provides an efficient approach for hiPSC differentiation into definitive endoderm and functional hepatocytes.
  • This method facilitates the study of molecular mechanisms underlying hepatic fate decisions.
  • The generated hepatocytes serve as a valuable surrogate cell source for pharmaceutical studies and drug discovery.