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Updated: Apr 20, 2026

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

Published on: May 2, 2014

Practical Methods for Molecular In Vivo Optical Imaging.

Hannah Chen¹, Stephen H Thorne¹

¹Departments of Surgery and Immunology, University of Pittsburgh Cancer Institute University of Pittsburgh, Pittsburgh, Pennsylvania, Phone 412 623 4896, Fax 412 623 7709.

Current Protocols in Cytometry

|November 25, 2014

Summary

This summary is machine-generated.

Optical imaging allows for real-time, whole-animal analysis, reducing animal use and labor. This technique enables rapid translation of cellular models into living animals, particularly rodents, for molecular event studies.

Keywords:

Bioluminescence fluorescence molecular imaging non-invasive reporter whole animal

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Area of Science:

Preclinical research
In vivo imaging
Molecular biology

Background:

Traditional methods for studying molecular events in vivo are labor-intensive and require sacrificing animals.
Subjective observations and measurements in live animals over time present challenges in quantitative analysis.
Advancements in animal imaging technologies offer new possibilities for real-time, quantitative studies.

Purpose of the Study:

To outline a streamlined process for translating in vitro cell models into in vivo rodent models.
To highlight the benefits of optical imaging for preclinical research and animal studies.
To enable researchers to conduct quantitative, real-time experiments with reduced animal numbers.

Main Methods:

Utilizing advanced optical imaging modalities, including bioluminescence and fluorescence imaging.
Adapting clinical imaging devices for dedicated small animal imaging applications.
Developing protocols for translating cell-based assays into rodent models for whole-animal analysis.

Main Results:

Optical imaging provides a powerful, cost-effective tool for in vivo studies.
Real-time, quantitative, whole-animal analysis is achievable with reduced experimental complexity.
Fewer animals are required compared to traditional sacrifice-based methodologies.

Conclusions:

Optical imaging significantly enhances the efficiency and scope of preclinical research.
This approach facilitates the rapid translation of cellular findings into living animal models.
Researchers can gain deeper insights into molecular events in a physiological context.