Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.9K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.9K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.7K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.7K
IR Spectrometers01:25

IR Spectrometers

3.6K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
3.6K
X-ray Imaging01:24

X-ray Imaging

11.2K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
11.2K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

1.0K
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
1.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surface-Enhanced Raman Spectroscopy to Characterize Different Fractions of Extracellular Vesicles from Control and Prostate Cancer Patients.

Biomedicines·2021
Same author

Monitoring Deuterium Uptake in Single Bacterial Cells via Two-Dimensional Raman Correlation Spectroscopy.

Analytical chemistry·2021
Same author

SERS characterization of dopamine and <i>in situ</i> dopamine polymerization on silver nanoparticles.

Physical chemistry chemical physics : PCCP·2021
Same author

Comparison of functional and discrete data analysis regimes for Raman spectra.

Analytical and bioanalytical chemistry·2021
Same author

Morpho-molecular signal correlation between optical coherence tomography and Raman spectroscopy for superior image interpretation and clinical diagnosis.

Scientific reports·2021
Same author

Simple and rapid peptide nanoprobe biosensor for the detection of Legionellaceae.

The Analyst·2021

Related Experiment Video

Updated: Apr 20, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

809

Raman imaging with a fiber-coupled multichannel spectrograph.

Elmar Schmälzlin1, Benito Moralejo2, Monika Rutowska3

  • 1Leibniz-Institut für Astrophysik Potsdam (AIP), An der Sternwarte 16, Potsdam 14482, Germany. eschmaelzlin@aip.de.

Sensors (Basel, Switzerland)
|November 25, 2014
PubMed
Summary

This study introduces a novel technique for rapid chemical mapping using Raman spectroscopy. The new method captures entire Raman images in a single exposure, significantly reducing analysis time compared to traditional scanning approaches.

More Related Videos

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.6K
Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

5.1K

Related Experiment Videos

Last Updated: Apr 20, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

809
Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.6K
Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

5.1K

Area of Science:

  • Spectroscopy
  • Chemical Imaging
  • Materials Science

Background:

  • Spatially resolved Raman spectroscopy traditionally involves time-consuming step-by-step sample scanning.
  • Acquiring detailed chemical maps requires extensive data collection over extended periods.

Purpose of the Study:

  • To develop and demonstrate a technique for capturing entire Raman images in a single exposure.
  • To overcome the limitations of conventional scanning methods in Raman spectroscopy.

Main Methods:

  • A novel probe head with a 20 × 20 multimode fiber array was developed.
  • This probe head was coupled to a high-performance astronomy spectrograph via a microscope.
  • Raman scattering from samples was collected in a single exposure.

Main Results:

  • Entire Raman images were successfully captured without any scanning procedure.
  • High-potential demonstration of the technique using reference samples.
  • Rapid generation of complete chemical maps was achieved.

Conclusions:

  • The presented technique enables significantly faster chemical mapping than traditional methods.
  • This single-exposure approach offers a powerful new tool for Raman spectroscopy applications.
  • The method has broad potential for various scientific and industrial fields requiring chemical analysis.