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Related Experiment Video

Updated: Apr 20, 2026

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Published on: April 3, 2020

Efficient single-cell poration by microsecond laser pulses.

Qihui Fan¹, Wenqi Hu, Aaron T Ohta

¹Department of Mechanical Engineering, University of Hawaii at Manoa, 2540 Dole Street, Holmes Hall 302, Honolulu, USA. fanqihui@hawaii.edu.

|November 26, 2014

Summary

This summary is machine-generated.

Laser-induced microbubbles efficiently deliver molecules into cells. This method achieves high cell poration and viability for both small dyes and large plasmids, enabling targeted gene delivery.

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Area of Science:

Biotechnology
Cell Biology
Laser-based technologies

Background:

Cell membrane poration is crucial for molecular delivery.
Existing methods face limitations in efficiency and targeting.
Laser-induced methods offer potential for precise cell manipulation.

Purpose of the Study:

To investigate laser-induced microbubbles for efficient molecular delivery into NIH/3T3 fibroblasts.
To optimize operational modes for delivering different molecular sizes.
To evaluate the efficiency and viability of the laser-based cell poration system.

Main Methods:

Utilized microsecond laser pulses to generate microbubbles for cell membrane poration.
Employed a scanning-laser mode for delivering smaller molecules like FITC-Dextran dye.
Applied a fixed-laser mode for efficient delivery of larger molecules, such as GFP plasmids.
Assessed poration efficiency and cell viability.

Main Results:

Achieved 95.1 ± 3.0% poration efficiency and cell viability for FITC-Dextran dye delivery using the scanning-laser mode.
Demonstrated high transfection efficiency of 86.7 ± 3.3% for 5.7 kb GFP plasmid DNA using the fixed-laser mode.
Showcased precise targeting of single cells and arbitrary cellular patterning capabilities.

Conclusions:

Laser-induced microbubbles provide a highly efficient and viable method for molecular delivery into fibroblasts.
Dual operational modes allow for tailored delivery of both small and large molecules.
This technology enables precise, high-resolution, and patternable cell poration for advanced applications.